C7923
Sigma
Monoclonal Anti-CD44 antibody produced in mouse
clone A3D8, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD00164629 |
Description
| Antigen Background | The CD44 antigen has been studied under the following names: Pgp-1, Hermes antigen, ECM-III, H-CAM and HUTCH-1. It functions as a homing receptor. CD44 antigen is the cellular receptor for hyaluronic acid. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells. |
| Immunogen | circulating malignant human Sezary T cells. |
| Specificity | Recognizes the CD44 (80-95 kDa) human cell surface glycoprotein on B and T lymphocytes, monocytes, granulocytes and red blood cells. The epitope recognized by this antibody is sensitive to bromelain and other proteolytic enzymes. It is detectable in routine formalin-fixed, paraffin-embedded tissue. 5th Workshop: code no. BP406, S320 |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | A3D8 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 80-95 kDa |
| species reactivity | human |
| application(s) | flow cytometry: 5 μL using 1 × 106 cells |
| isotype | IgG1 |
| Gene Information | human ... CD44(960) |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 3 |
References
| reference | Haynes, B.F., et al. Immunology Today 10, 423, (1989) Abstract |
| Denning, S.M., et al. J. Immunol. 144, 7, (1990) Abstract | |
| Hale, L. P., et al. J. Immunol. 149, 3809, (1992) Abstract | |
| Ellerkamp, V., et al., De novo development of a cavernous malformation of the brain: significance of factors with paracrine and endocrine activity: case report. Neurosurgery 50, 646-649, (2002) Abstract | |
| Chabadel, A., et al., CD44 and β3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts Mol. Biol. Cell 18, 4899-4910, (2007) Abstract |
